NAD+ peptide calculator
A 500 mg NAD+ vial mixed with 10 ml of bacteriostatic water holds 50 mg/ml. A 100 mg dose is 2.00 ml — 200 units on a U-100 insulin syringe — and the vial holds 5 doses of that size.
NAD+ dosage chart: units to draw
Units on a U-100 insulin syringe (100 units = 1 ml) for NAD+ doses from 50 mg to 500 mg, by how much bacteriostatic water is in a 500 mg vial. The highlighted row is the 100 mg dose used above.
| Dose | 5 ml water 100 mg/ml | 10 ml water 50 mg/ml | 15 ml water 33.3 mg/ml | Doses per vial |
|---|---|---|---|---|
| 50 mg | 50 u 0.50 ml | 100 u 1.00 ml | 1.50 ml over 100 u | 10 |
| 100 mg | 100 u 1.00 ml | 2.00 ml over 100 u | 3.00 ml over 100 u | 5 |
| 200 mg | 2.00 ml over 100 u | 4.00 ml over 100 u | 6.00 ml over 100 u | 2 |
| 500 mg | 5.00 ml over 100 u | 10.00 ml over 100 u | 15.00 ml over 100 u | 1 |
At this mix a 100 mg NAD+ dose is 2.00 ml — more than a full U-100 insulin syringe (1 ml, 100 units). Volumes this large are usually drawn with a standard graduated syringe instead, or the dose is split.
Unit math only, not a dosing recommendation. The dose range is what published protocols and trials describe; see the NAD+ guide for sources.
How to reconstitute NAD+
- Wipe both vial stoppers with a fresh alcohol swab and let them dry.
- Draw 10 ml of bacteriostatic water into a syringe.
- Insert the needle at an angle into the NAD+ vial and let the water run slowly down the inner wall — do not inject directly onto the powder.
- Do not shake. Gently swirl the vial until the peptide fully dissolves.
- Storage for NAD+: Lyophilized: refrigerated. Reconstituted: refrigerated, use within 7–14 days (shorter than most peptides).
- For a 100 mg dose, draw to the 200-unit mark (2.00 ml) on a U-100 insulin syringe.
About NAD+
NAD+ (nicotinamide adenine dinucleotide) is an endogenous redox cofactor central to mitochondrial energy production, sirtuin activity, and DNA-repair signaling. Parenteral NAD+ is administered in anti-aging and addiction-recovery research protocols.
Half-life: Approximately 4–10 minutes (IV); longer for subcutaneous
Typical dose range: 100–500 mg per IV infusion; 50–200 mg per subcutaneous injection
Class: Cofactor / redox coenzyme
Regulatory status: Not FDA approved for anti-aging or longevity uses. Compounded NAD+ is available through 503A pharmacies in some states.
Read the full NAD+ guide → — mechanism, side effects, storage, regulatory status and citations. Or browse the whole peptide glossary.
Other tools for NAD+
Calculators for related peptides: SS-31, Epithalon, MOTS-c.
Need a different unit or a different question? The peptide dose converter moves a dose between mcg, mg and IU, and the half-life visualizer shows how concentration accumulates across a dosing cadence. Loading NAD+ into a reusable 3 ml pen instead of drawing it with a syringe? The peptide pen calculator turns the same vial into mcg per click and clicks to dial. All of them are listed on the free peptide tools page.
NAD+ calculator FAQ
Is this a NAD+ peptide calculator or a reconstitution calculator?
Both. This NAD+ calculator converts vial strength, bacteriostatic-water volume, and target dose into milliliters and insulin-syringe units. It does not recommend a medical dose.
How much bacteriostatic water for a 500 mg NAD+ vial?
This page is pre-filled with 10 ml of bacteriostatic water for a 500 mg NAD+ vial because that is a common research ratio. You can adjust the water amount in the full calculator.
How many units is a 100 mg NAD+ dose?
200 units on a U-100 insulin syringe, when a 500 mg vial is mixed with 10 ml of bacteriostatic water (50 mg/ml). That is 2.00 ml. With a different amount of water the unit count changes — the dose chart on this page lists 5 ml, 10 ml, 15 ml mixes.
How many doses are in a 500 mg NAD+ vial?
5 doses of 100 mg. The number of doses depends only on vial strength and dose size — adding more or less water changes the volume of each draw, not how many draws the vial holds.
Does this NAD+ dosage calculator give medical advice?
No. It only performs unit math. Dosing, titration, and whether NAD+ is appropriate are clinical decisions that should be made with a licensed healthcare professional.
Skip the math — let the app handle it
Peptide Protocol remembers your NAD+ vial, shows the units for every dose, tracks what is left, and rotates injection sites for you.
Get the iPhone app →Educational use only. Peptide Protocol is an informational tool. Nothing on this page constitutes medical advice. Many peptides are prescription-only or restricted in your jurisdiction. Always consult a licensed healthcare professional before injecting any compound.