SS-31 peptide calculator
A 10 mg SS-31 vial mixed with 2 ml of bacteriostatic water holds 5 mg/ml. A 2 mg dose is 0.40 ml — 40 units on a U-100 insulin syringe — and the vial holds 5 doses of that size.
SS-31 dosage chart: units to draw
Units on a U-100 insulin syringe (100 units = 1 ml) for SS-31 doses from 1 mg to 4 mg, by how much bacteriostatic water is in a 10 mg vial. The highlighted row is the 2 mg dose used above.
| Dose | 1 ml water 10 mg/ml | 2 ml water 5 mg/ml | 3 ml water 3.33 mg/ml | Doses per vial |
|---|---|---|---|---|
| 1 mg | 10 u 0.10 ml | 20 u 0.20 ml | 30 u 0.30 ml | 10 |
| 2 mg | 20 u 0.20 ml | 40 u 0.40 ml | 60 u 0.60 ml | 5 |
| 3 mg | 30 u 0.30 ml | 60 u 0.60 ml | 90 u 0.90 ml | 3 |
| 4 mg | 40 u 0.40 ml | 80 u 0.80 ml | 1.20 ml over 100 u | 2 |
A 40-unit draw fits a 0.5 ml insulin syringe (50-unit capacity) with 1-unit markings.
Unit math only, not a dosing recommendation. The dose range is what published protocols and trials describe; see the SS-31 guide for sources.
How to reconstitute SS-31
- Wipe both vial stoppers with a fresh alcohol swab and let them dry.
- Draw 2 ml of bacteriostatic water into a syringe.
- Insert the needle at an angle into the SS-31 vial and let the water run slowly down the inner wall — do not inject directly onto the powder.
- Do not shake. Gently swirl the vial until the peptide fully dissolves.
- Storage for SS-31: Lyophilized refrigerated. Reconstituted: refrigerated 2–8 °C, use within 28 days.
- For a 2 mg dose, draw to the 40-unit mark (0.40 ml) on a U-100 insulin syringe.
About SS-31
SS-31 (elamipretide) is a synthetic tetrapeptide that selectively concentrates in mitochondria, binds cardiolipin on the inner membrane, and stabilizes mitochondrial structure and function.
Half-life: Approximately 4 hours (subcutaneous)
Typical dose range: 2–40 mg (2,000–40,000 mcg) per day in clinical trials; research protocols vary widely
Class: Mitochondria-targeted tetrapeptide
Regulatory status: Not FDA approved. In ongoing clinical trials for primary mitochondrial myopathy, Barth syndrome, and age-related macular degeneration.
Read the full SS-31 guide → — mechanism, side effects, storage, regulatory status and citations. Or browse the whole peptide glossary.
Other tools for SS-31
Calculators for related peptides: NAD+, Epithalon, MOTS-c.
Need a different unit or a different question? The peptide dose converter moves a dose between mcg, mg and IU, and the half-life visualizer shows how concentration accumulates across a dosing cadence. Loading SS-31 into a reusable 3 ml pen instead of drawing it with a syringe? The peptide pen calculator turns the same vial into mcg per click and clicks to dial. All of them are listed on the free peptide tools page.
SS-31 calculator FAQ
Is this a SS-31 peptide calculator or a reconstitution calculator?
Both. This SS-31 calculator converts vial strength, bacteriostatic-water volume, and target dose into milliliters and insulin-syringe units. It does not recommend a medical dose.
How much bacteriostatic water for a 10 mg SS-31 vial?
This page is pre-filled with 2 ml of bacteriostatic water for a 10 mg SS-31 vial because that is a common research ratio. You can adjust the water amount in the full calculator.
How many units is a 2 mg SS-31 dose?
40 units on a U-100 insulin syringe, when a 10 mg vial is mixed with 2 ml of bacteriostatic water (5 mg/ml). That is 0.40 ml. With a different amount of water the unit count changes — the dose chart on this page lists 1 ml, 2 ml, 3 ml mixes.
How many doses are in a 10 mg SS-31 vial?
5 doses of 2 mg. The number of doses depends only on vial strength and dose size — adding more or less water changes the volume of each draw, not how many draws the vial holds.
Does this SS-31 dosage calculator give medical advice?
No. It only performs unit math. Dosing, titration, and whether SS-31 is appropriate are clinical decisions that should be made with a licensed healthcare professional.
Skip the math — let the app handle it
Peptide Protocol remembers your SS-31 vial, shows the units for every dose, tracks what is left, and rotates injection sites for you.
Get the iPhone app →Educational use only. Peptide Protocol is an informational tool. Nothing on this page constitutes medical advice. Many peptides are prescription-only or restricted in your jurisdiction. Always consult a licensed healthcare professional before injecting any compound.