Semaglutide peptide calculator
A 5 mg Semaglutide vial mixed with 2 ml of bacteriostatic water holds 2.5 mg/ml. A 250 mcg dose is 0.10 ml — 10 units on a U-100 insulin syringe — and the vial holds 20 doses of that size.
Semaglutide dosage chart: units to draw
Units on a U-100 insulin syringe (100 units = 1 ml) for Semaglutide doses from 250 mcg to 2.4 mg, by how much bacteriostatic water is in a 5 mg vial. The highlighted row is the 250 mcg dose used above.
| Dose | 1 ml water 5 mg/ml | 2 ml water 2.5 mg/ml | 3 ml water 1.67 mg/ml | Doses per vial |
|---|---|---|---|---|
| 250 mcg | 5 u 0.050 ml | 10 u 0.10 ml | 15 u 0.15 ml | 20 |
| 500 mcg | 10 u 0.10 ml | 20 u 0.20 ml | 30 u 0.30 ml | 10 |
| 1 mg | 20 u 0.20 ml | 40 u 0.40 ml | 60 u 0.60 ml | 5 |
| 1.7 mg | 34 u 0.34 ml | 68 u 0.68 ml | 1.02 ml over 100 u | 2 |
| 2.4 mg | 48 u 0.48 ml | 96 u 0.96 ml | 1.44 ml over 100 u | 2 |
A 10-unit draw fits comfortably on a 0.3 ml insulin syringe (30-unit capacity), whose 1-unit (often half-unit) markings are easier to read than the 2-unit ticks on a 1 ml syringe.
Unit math only, not a dosing recommendation. The dose range is what published protocols and trials describe; see the Semaglutide guide for sources.
How to reconstitute Semaglutide
- Wipe both vial stoppers with a fresh alcohol swab and let them dry.
- Draw 2 ml of bacteriostatic water into a syringe.
- Insert the needle at an angle into the Semaglutide vial and let the water run slowly down the inner wall — do not inject directly onto the powder.
- Do not shake. Gently swirl the vial until the peptide fully dissolves.
- Storage for Semaglutide: Lyophilized stable refrigerated. Reconstituted: refrigerated 2–8 °C, use within 28 days.
- For a 250 mcg dose, draw to the 10-unit mark (0.10 ml) on a U-100 insulin syringe.
About Semaglutide
Semaglutide is a long-acting GLP-1 receptor agonist approved for type 2 diabetes and chronic weight management. It slows gastric emptying, enhances insulin secretion, and reduces appetite.
Half-life: Approximately 7 days (once-weekly dosing)
Typical dose range: Titration: 0.25 mg (250 mcg) → 0.5 mg → 1.0 mg → 1.7 mg → 2.4 mg weekly
Class: GLP-1 receptor agonist
Regulatory status: FDA approved as Ozempic (type 2 diabetes), Wegovy (obesity), Rybelsus (oral T2D). Compounded semaglutide is not FDA approved and is under active FDA scrutiny.
Read the full Semaglutide guide → — mechanism, side effects, storage, regulatory status and citations. Or browse the whole peptide glossary.
Other tools for Semaglutide
Calculators for related peptides: Liraglutide, Retatrutide, Survodutide, Tirzepatide, AOD-9604.
Need a different unit or a different question? The peptide dose converter moves a dose between mcg, mg and IU, and the Semaglutide half-life chart shows how concentration accumulates across a dosing cadence. Loading Semaglutide into a reusable 3 ml pen instead of drawing it with a syringe? The peptide pen calculator turns the same vial into mcg per click and clicks to dial. All of them are listed on the free peptide tools page.
Semaglutide calculator FAQ
Is this a Semaglutide peptide calculator or a reconstitution calculator?
Both. This Semaglutide calculator converts vial strength, bacteriostatic-water volume, and target dose into milliliters and insulin-syringe units. It does not recommend a medical dose.
How much bacteriostatic water for a 5 mg Semaglutide vial?
This page is pre-filled with 2 ml of bacteriostatic water for a 5 mg Semaglutide vial because that is a common research ratio. You can adjust the water amount in the full calculator.
How many units is a 250 mcg Semaglutide dose?
10 units on a U-100 insulin syringe, when a 5 mg vial is mixed with 2 ml of bacteriostatic water (2.5 mg/ml). That is 0.10 ml. With a different amount of water the unit count changes — the dose chart on this page lists 1 ml, 2 ml, 3 ml mixes.
How many doses are in a 5 mg Semaglutide vial?
20 doses of 250 mcg. The number of doses depends only on vial strength and dose size — adding more or less water changes the volume of each draw, not how many draws the vial holds.
Does this Semaglutide dosage calculator give medical advice?
No. It only performs unit math. Dosing, titration, and whether Semaglutide is appropriate are clinical decisions that should be made with a licensed healthcare professional.
Skip the math — let the app handle it
Peptide Protocol remembers your Semaglutide vial, shows the units for every dose, tracks what is left, and rotates injection sites for you.
Get the iPhone app →Educational use only. Peptide Protocol is an informational tool. Nothing on this page constitutes medical advice. Many peptides are prescription-only or restricted in your jurisdiction. Always consult a licensed healthcare professional before injecting any compound.