TB-500 peptide calculator
A 10 mg TB-500 vial mixed with 3 ml of bacteriostatic water holds 3.33 mg/ml. A 2 mg dose is 0.60 ml — 60 units on a U-100 insulin syringe — and the vial holds 5 doses of that size.
TB-500 dosage chart: units to draw
Units on a U-100 insulin syringe (100 units = 1 ml) for TB-500 doses from 1 mg to 5 mg, by how much bacteriostatic water is in a 10 mg vial. The highlighted row is the 2 mg dose used above.
| Dose | 1 ml water 10 mg/ml | 2 ml water 5 mg/ml | 3 ml water 3.33 mg/ml | Doses per vial |
|---|---|---|---|---|
| 1 mg | 10 u 0.10 ml | 20 u 0.20 ml | 30 u 0.30 ml | 10 |
| 2 mg | 20 u 0.20 ml | 40 u 0.40 ml | 60 u 0.60 ml | 5 |
| 3 mg | 30 u 0.30 ml | 60 u 0.60 ml | 90 u 0.90 ml | 3 |
| 4 mg | 40 u 0.40 ml | 80 u 0.80 ml | 1.20 ml over 100 u | 2 |
| 5 mg | 50 u 0.50 ml | 100 u 1.00 ml | 1.50 ml over 100 u | 2 |
A 60-unit draw needs a full 1 ml insulin syringe (100-unit capacity). Using less water concentrates the vial and shrinks the draw.
Unit math only, not a dosing recommendation. The dose range is what published protocols and trials describe; see the TB-500 guide for sources.
How to reconstitute TB-500
- Wipe both vial stoppers with a fresh alcohol swab and let them dry.
- Draw 3 ml of bacteriostatic water into a syringe.
- Insert the needle at an angle into the TB-500 vial and let the water run slowly down the inner wall — do not inject directly onto the powder.
- Do not shake. Gently swirl the vial until the peptide fully dissolves.
- Storage for TB-500: Lyophilized stable at room temp. Reconstituted: refrigerated, use within 28 days.
- For a 2 mg dose, draw to the 60-unit mark (0.60 ml) on a U-100 insulin syringe.
About TB-500
TB-500 is a synthetic peptide fragment based on the active region of thymosin beta-4, a naturally occurring protein involved in cell migration, angiogenesis, and wound healing.
Half-life: Approximately 2 hours (peptide); biological effect lasts longer
Typical dose range: 2–5 mg (2000–5000 mcg) per injection, 1–2x weekly loading, then maintenance
Class: Synthetic peptide fragment of thymosin beta-4
Regulatory status: Not FDA approved. Research use only in the US.
Read the full TB-500 guide → — mechanism, side effects, storage, regulatory status and citations. Or browse the whole peptide glossary.
Other tools for TB-500
Calculators for related peptides: BPC-157, GHK-Cu.
Need a different unit or a different question? The peptide dose converter moves a dose between mcg, mg and IU, and the TB-500 half-life chart shows how concentration accumulates across a dosing cadence. Loading TB-500 into a reusable 3 ml pen instead of drawing it with a syringe? The peptide pen calculator turns the same vial into mcg per click and clicks to dial. All of them are listed on the free peptide tools page.
TB-500 calculator FAQ
Is this a TB-500 peptide calculator or a reconstitution calculator?
Both. This TB-500 calculator converts vial strength, bacteriostatic-water volume, and target dose into milliliters and insulin-syringe units. It does not recommend a medical dose.
How much bacteriostatic water for a 10 mg TB-500 vial?
This page is pre-filled with 3 ml of bacteriostatic water for a 10 mg TB-500 vial because that is a common research ratio. You can adjust the water amount in the full calculator.
How many units is a 2 mg TB-500 dose?
60 units on a U-100 insulin syringe, when a 10 mg vial is mixed with 3 ml of bacteriostatic water (3.33 mg/ml). That is 0.60 ml. With a different amount of water the unit count changes — the dose chart on this page lists 1 ml, 2 ml, 3 ml mixes.
How many doses are in a 10 mg TB-500 vial?
5 doses of 2 mg. The number of doses depends only on vial strength and dose size — adding more or less water changes the volume of each draw, not how many draws the vial holds.
Does this TB-500 dosage calculator give medical advice?
No. It only performs unit math. Dosing, titration, and whether TB-500 is appropriate are clinical decisions that should be made with a licensed healthcare professional.
Skip the math — let the app handle it
Peptide Protocol remembers your TB-500 vial, shows the units for every dose, tracks what is left, and rotates injection sites for you.
Get the iPhone app →Educational use only. Peptide Protocol is an informational tool. Nothing on this page constitutes medical advice. Many peptides are prescription-only or restricted in your jurisdiction. Always consult a licensed healthcare professional before injecting any compound.